Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: The degradation of RS by B. adolescentis P2P3. Optical microscopic images of a dispersed RS granules without cells, b clustered RS granules immediately after inoculation of cells, c slightly degraded RS granule clusters after 12 h of incubation, d, e fairly degraded RS granule clusters after 24 h and 32 h, respectively, and f almost degraded RS granule clusters after 48 h

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques: Incubation

Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: The RS-degradation characteristics of six B. adolescentis strains

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques:

Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: Visualized circular map of chromosome of B. adolescentis P2P3. 2.2 Mbp of chromosome consists of seven tracks. From the outermost, track 1 (deep blue): forward-strand coding genes, track 2 (blue): reverse-strand coding genes, track 3 (light blue): tRNAs, track 4 (Orange): rRNAs, track 5 (black): GC content, and track 6 (green and purple): G+C skew

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques:

Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: Genome characteristics of six B. adolescentis strains

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques: CRISPR

Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: Relationship between B. adolescentis strains based on sequence alignment. The number in the box indicates percent identity. a Phylogenetic analysis based on 16S rDNA. The bar represents 0.0005 substitutions per site. b Genome distance analysis (ANI). The number in the tree represents the branch length

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques: Sequencing

Journal: 3 Biotech

Article Title: Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

doi: 10.1007/s13205-019-2019-7

Figure Lengend Snippet: The genes involved in the hydrolysis of α-1,4 and 1,6-glucosidic linkages in B. adolescentis P2P3

Article Snippet: Bifidobacterium adolescentis strains Bifidobacterium adolescentis strains (DSM 20083, DSM 20086, DSM 20087, and DSM 24849), were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and B. adolescentis L2-32 was kindly provided from BEI resources (Manassas, VA, USA).

Techniques:

Journal: Pharmaceutics

Article Title: Functional and Safety Profile of Limosilactobacillus vaginalis and Development of Oral Fast-Disintegrating Tablets for Gut Microbiota Modulation

doi: 10.3390/pharmaceutics17081011

Figure Lengend Snippet: Effect of L. vaginalis BC17 fractions (viable BC17, CFS-BC17, HK-BC17, viable+CFS-BC17 and HK+CFS-BC17) on Bifidobacterium spp. adhesion to Caco-2 cells. ( A ) Adherent Bifidobacterium per Caco-2 cell after 3 h of co-incubation (mean ± SD, n = 3), * p < 0.05 (vs. control). ( B ) Boxplot of Bifidobacterium adhesion percentage compared to the control (100%). Each box represents the interquartile range (25–75th percentile). Lines within the boxes indicate the median values of the samples. The extremes of the bars indicate the minimum and maximum values, respectively. Statistically significant differences between the samples are indicated by different letters ( p < 0.05).

Article Snippet: Bifidobacterium strains ( B. breve DSM 20091, B. breve DSM 20456, B. bifidum DSM 20082, B. bifidum DSM 20213, B. bifidum DSM 20215, B. longum subsp. longum DSM 20219, B. longum subsp. infantis DSM 20088, B. longum subsp. infantis DSM 20090, B. adolescentis DSM 20083, B. adolescentis DSM 20086, B. angulatum DSM 20098) were purchased from DSMZ [ ].

Techniques: Incubation, Control

Journal: Pharmaceutics

Article Title: Functional and Safety Profile of Limosilactobacillus vaginalis and Development of Oral Fast-Disintegrating Tablets for Gut Microbiota Modulation

doi: 10.3390/pharmaceutics17081011

Figure Lengend Snippet: Functional characterization of tablets (Tab HK+CFS-BC17 and Tab viable+CFS-BC17). Tablets were tested immediately after the preparation (T0) and after 3 months of storage at room temperature (T3). ( A ) Effects on viability of Caco-2 and HT-29 cells after 24 h and 48 h of incubation, evaluated by MTT assay and dye exclusion test. Overall results are presented as boxplot reporting the increase of cell viability (%) compared to the control (dotted line). ( B ) Effects on Bifidobacterium spp. adhesion to Caco-2 cells. Boxplot reported the percentages compared to the control (100%). ( C ) Protective effects on Caco-2 and HT-29 cells exposed to SDS 0.05% for 24 h and 48 h. Overall results are presented as boxplot reporting the increase of cell viability (%) compared to cells exposed to SDS (dotted line). Each box represents the interquartile range (25–75th percentile). Lines within the boxes indicate the median values of the samples. The extremes of the bars indicate the minimum and maximum values, respectively. ( D ) Inhibitory effect on NO production in LPS-induced macrophages. Results are in percentages with respect to control (means ± SD). All tests were performed in triplicate; statistically significant differences between the samples are indicated by different letters ( p < 0.05).

Article Snippet: Bifidobacterium strains ( B. breve DSM 20091, B. breve DSM 20456, B. bifidum DSM 20082, B. bifidum DSM 20213, B. bifidum DSM 20215, B. longum subsp. longum DSM 20219, B. longum subsp. infantis DSM 20088, B. longum subsp. infantis DSM 20090, B. adolescentis DSM 20083, B. adolescentis DSM 20086, B. angulatum DSM 20098) were purchased from DSMZ [ ].

Techniques: Functional Assay, Incubation, MTT Assay, Control

Journal: Pharmaceutics

Article Title: Functional and Safety Profile of Limosilactobacillus vaginalis and Development of Oral Fast-Disintegrating Tablets for Gut Microbiota Modulation

doi: 10.3390/pharmaceutics17081011

Figure Lengend Snippet: L. vaginalis BC17 adhesion to Caco-2 cells co-incubated with Bifidobacterium spp. and enteropathogens. The adhesion of L. vaginalis BC17 without test microorganisms (BC17 alone) was evaluated for comparison as follows: viable BC17 at ratios of 100:1 and 33:1 with Caco-2 cells, and viable BC17 in the presence of CFS (viable+CFS-BC17). ( A ) Results are reported as adherent BC17 per Caco-2 cell for L. vaginalis in the presence of bifidobacteria, enterotoxigenic E. coli (ETEC), S. enterica or Y. enterocolitica (mean ± SD, n = 3), * p < 0.05 (viable BC17+ test microorganisms vs. BC17 alone 100:1), # p < 0.05 (viable+CFS-BC17 + test microorganisms vs. viable+CFS-BC17 alone). ( B ) Boxplot of adherent BC17 per Caco-2 cell. Lines within the boxes indicate the median values of the samples. The extremes of the bars indicate the minimum and maximum values, respectively. Statistically significant differences between the samples are indicated by different letters ( p < 0.05).

Article Snippet: Bifidobacterium strains ( B. breve DSM 20091, B. breve DSM 20456, B. bifidum DSM 20082, B. bifidum DSM 20213, B. bifidum DSM 20215, B. longum subsp. longum DSM 20219, B. longum subsp. infantis DSM 20088, B. longum subsp. infantis DSM 20090, B. adolescentis DSM 20083, B. adolescentis DSM 20086, B. angulatum DSM 20098) were purchased from DSMZ [ ].

Techniques: Incubation, Comparison